Response: Commentary: Analysis of SUMO1-conjugation at synapses

Commentary: Analysis of SUMO1-conjugation at synapses

Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, IPMC, Université Côte d’Azur, Nice, France, 3 Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland, 4 Taub Institute and Department of Pathology and Cell Biology, Columbia University, New York, NY, United States, 5 Department of Applied Sciences, University of the West of England, B...

Analysis of SUMO1-conjugation at synapses

SUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and immunostaining analyses of synaptic SUMO1-conjugation. By using a knock-in repor...

Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential.

The posttranslational addition of small ubiquitin-like modifiers (SUMOs) to other intracellular proteins has been implicated in a variety of eukaryotic functions, including modifying cytoplasmic signal transduction, nuclear import and subnuclear compartmentalization, DNA repair, and transcription regulation. For plants, in particular, both genetic analyses and the rapid accumulation of SUMO con...

Comparison of the SUMO1 and ubiquitin conjugation pathways during the inhibition of proteasome activity with evidence of SUMO1 recycling.

To investigate potential interplay between the SUMO1 (small ubiquitin-related modifier-1) and ubiquitin pathways of post-translational protein modification, we examined aspects of their localization and conjugation status during proteasome inhibition. Our results indicate that these pathways converge upon the discrete sub-nuclear domains known as PML (promyelocytic leukaemia protein) NBs (nucle...

Commentary: GARLH Family Proteins Stabilize GABAA Receptors at Synapses